Fischer R, König K, Rück A, Puhl W, Steiner R
Orthopädische Klinik und Querschnittgelähmtenzentrum, Universität Ulm.
Z Orthop Ihre Grenzgeb. 1994 Jan-Feb;132(1):9-15. doi: 10.1055/s-2008-1039814.
Our intention was to test fluorescence spectroscopy as a possibility for selective laser ablation. In an experimental setup a XeCl excimer laser (308 nm) with pulse energies between 5 and 50 mJ was used for fluorescence excitation in 20 human and 100 pig vertebral segments. Tissue fluorescence was detected via a quartz fiber bundle and analyzed by a polychromator and optical multichannel analyzer. Both low and high energy levels led to a broad band fluorescence of nucleus pulposus, intervertebral muscle, spinal cord and vertebral end plate and a typical fluorescence of anulus fibrosus with peak maxima at 385 and 435 nm. Tissue fluorescence was independent of surrounding medium (with air and normal saline used) and excitation energy. Borderlines between different tissues could be clearly indicated in all cases. Using intensity rations at 350, 385 and 435 nm (I1 = I350/I385, I2 = I385/I435) tissue discrimination was possible in all cases. This results demonstrate, that discrimination between intervertebral disc and surrounding tissue is possible and could be used for selective percutaneous laser nucleotomy in the near future.
我们的目的是测试荧光光谱法作为选择性激光消融的一种可能性。在一个实验装置中，使用一台脉冲能量在5至50毫焦之间的XeCl准分子激光（308纳米）对20个人类和100个猪的椎骨节段进行荧光激发。通过石英纤维束检测组织荧光，并由多色仪和光学多通道分析仪进行分析。低能量水平和高能量水平都会导致髓核、椎间肌肉、脊髓和椎体终板产生宽带荧光，以及纤维环产生典型荧光，其峰值最大值分别在385纳米和435纳米处。组织荧光与周围介质（使用空气和生理盐水）以及激发能量无关。在所有情况下，不同组织之间的边界都能清晰显示。利用350纳米、385纳米和435纳米处的强度比（I1 = I350/I385，I2 = I385/I435），在所有情况下都能够进行组织鉴别。这些结果表明，椎间盘与周围组织之间的鉴别是可行的，并且在不久的将来可用于选择性经皮激光髓核摘除术。
