Isaka T, Yoshimine T, Maruno M, Kuroda R, Ishii H, Hayakawa T
Department of Neurosurgery, Osaka University Medical School, Japan.
Acta Neuropathol. 1994;87(1):81-5. doi: 10.1007/BF00386257.
A total of 14 surgical specimens, including 7 glioblastomas, 3 anaplastic astrocytomas, 2 brains adjacent to glioblastoma and 2 grossly normal brains, were investigated immunohistochemically for the expression of antithrombin III (AT-III), heparan sulfate proteoglycan (HSPG) and thrombomodulin (TM) in the endothelium of microvessels. The immunoreaction to AT-III was of moderate intensity in grossly normal brains, brains adjacent to glioblastoma, and anaplastic astrocytomas, but was only weak in glioblastomas, especially in the capillaries. The immunoreaction to HSPG was constantly intense in the microvessels in all the specimens. Although the immunoreaction to TM was negative or only faint in the microvessels in grossly normal brains, it was moderately to strongly intense in anaplastic astrocytomas and brains adjacent to glioblastoma. The intensity of immunoreaction to TM was variable, from faint to strong in the capillaries, and moderate to strong in larger microvessels in glioblastomas. The present study suggested that the alterations in the expression of those antithrombotic molecules could explain, at least in part, the tendencies for intratumoral hemorrhage as well as intravascular thrombosis in the different areas of malignant gliomas.
总共14个手术标本，包括7个胶质母细胞瘤、3个间变性星形细胞瘤、2个与胶质母细胞瘤相邻的脑组织以及2个大体正常的脑组织，进行了免疫组织化学研究，以检测微血管内皮中抗凝血酶III（AT-III）、硫酸乙酰肝素蛋白聚糖（HSPG）和血栓调节蛋白（TM）的表达。在大体正常的脑组织、与胶质母细胞瘤相邻的脑组织以及间变性星形细胞瘤中，对AT-III的免疫反应强度适中，但在胶质母细胞瘤中较弱，尤其是在毛细血管中。在所有标本的微血管中，对HSPG的免疫反应持续强烈。虽然在大体正常的脑组织的微血管中对TM的免疫反应为阴性或仅微弱，但在间变性星形细胞瘤和与胶质母细胞瘤相邻的脑组织中为中度至强烈。在胶质母细胞瘤中，对TM的免疫反应强度在毛细血管中从微弱到强烈不等，在较大的微血管中为中度至强烈。本研究表明，这些抗血栓分子表达的改变至少可以部分解释恶性胶质瘤不同区域的肿瘤内出血以及血管内血栓形成的倾向。
