巨大脱硫弧菌含钼醛氧化还原酶基因的分子克隆及序列分析。推导的氨基酸序列与黄嘌呤脱氢酶具有相似性。
Molecular cloning and sequence analysis of the gene of the molybdenum-containing aldehyde oxido-reductase of Desulfovibrio gigas. The deduced amino acid sequence shows similarity to xanthine dehydrogenase.
作者信息
Thoenes U, Flores O L, Neves A, Devreese B, Van Beeumen J J, Huber R, Romão M J, LeGall J, Moura J J, Rodrigues-Pousada C
机构信息
Instituto Gulbekian de Ciênca, Laboratório de Genética Molecular, Oeiras, Portugal.
出版信息
Eur J Biochem. 1994 Mar 15;220(3):901-10. doi: 10.1111/j.1432-1033.1994.tb18693.x.
In this report, we describe the isolation of a 4020-bp genomic PstI fragment of Desulfovibrio gigas harboring the aldehyde oxido-reductase gene. The aldehyde oxido-reductase gene spans 2718 bp of genomic DNA and codes for a protein with 906 residues. The protein sequence shows an average 52% (+/- 1.5%) similarity to xanthine dehydrogenase from different organisms. The codon usage of the aldehyde oxidoreductase is almost identical to a calculated codon usage of the Desulfovibrio bacteria.
在本报告中，我们描述了从巨大脱硫弧菌中分离出一个携带醛氧化还原酶基因的4020 bp基因组PstI片段。醛氧化还原酶基因跨度为2718 bp的基因组DNA，编码一个含有906个残基的蛋白质。该蛋白质序列与来自不同生物体的黄嘌呤脱氢酶平均相似度为52%（±1.5%）。醛氧化还原酶的密码子使用情况与脱硫弧菌计算出的密码子使用情况几乎相同。
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