Shukovski L, Dyson M, Findlay J K
Prince Henry's Institute of Medical Research, Clayton, Victoria, Australia.
Mol Cell Endocrinol. 1993 Nov;97(1-2):19-27. doi: 10.1016/0303-7207(93)90207-z.
The paracrine actions of bovine follistatin (FS), human recombinant activin A and bovine inhibin on progesterone (P), androstenedione (A4) and inhibin production, were investigated using LH-stimulated immature bovine thecal cells. The presence of FS (3-100 ng/ml) alone caused a dose-dependent stimulation of P production by thecal cells induced by bovine LH (10 ng/ml). The stimulatory effect of FS on P production at 10 or 30 ng/ml was reversed to control levels with the addition of activin (10 or 30 ng/ml). Treatment with FS did not significantly effect on A4 production. Activin alone had no consistent effect on A4 production (measured using two different antibodies), but had a dose-dependent inhibitory effect on P production. Treatments of cells with inhibin had no significant effect on the LH-induced production of either P or A4. Testosterone production in FS; activin- or inhibin-treated cells was not different from controls. Northern analysis showed that inhibin beta subunit was not detected in thecal mRNA, whereas there were very faint bands of inhibin alpha subunit and FS which were attributed to contamination of granulosa cells (GC). We conclude that FS in vitro has a stimulatory effect on P production by bovine thecal cells, and that activin has the ability to reverse the stimulatory effect of P production. Unlike the rat and human thecal cells, activin and inhibin had no significant effect on LH-induced androgen synthesis by bovine thecal cells. We propose that FS secreted by the GC acts as a paracrine modulator upon thecal cells to directly stimulate the production of P independently of activin.(ABSTRACT TRUNCATED AT 250 WORDS)
利用促黄体生成素（LH）刺激的未成熟牛卵泡膜细胞，研究了牛卵泡抑素（FS）、重组人激活素A和牛抑制素对孕酮（P）、雄烯二酮（A4）分泌及抑制素产生的旁分泌作用。单独存在FS（3 - 100 ng/ml）时，可引起牛LH（10 ng/ml）诱导的卵泡膜细胞P分泌呈剂量依赖性增加。加入激活素（10或30 ng/ml）后，10或30 ng/ml FS对P分泌的刺激作用恢复至对照水平。FS处理对A4分泌无显著影响。单独使用激活素对A4分泌（使用两种不同抗体检测）无一致影响，但对P分泌有剂量依赖性抑制作用。用抑制素处理细胞对LH诱导的P或A4分泌无显著影响。FS、激活素或抑制素处理的细胞中睾酮分泌与对照无差异。Northern分析显示，在卵泡膜细胞mRNA中未检测到抑制素β亚基，而抑制素α亚基和FS的条带非常微弱，归因于颗粒细胞（GC）污染。我们得出结论，体外FS对牛卵泡膜细胞P分泌有刺激作用，激活素能够逆转P分泌的刺激作用。与大鼠和人类卵泡膜细胞不同，激活素和抑制素对牛卵泡膜细胞LH诱导的雄激素合成无显著影响。我们提出，颗粒细胞分泌的FS作为旁分泌调节剂作用于卵泡膜细胞，独立于激活素直接刺激P的产生。（摘要截短至250字）
