Beall B, Moran C P
Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322.
J Bacteriol. 1994 Apr;176(7):2003-12. doi: 10.1128/jb.176.7.2003-2012.1994.
Screening for sigma E-dependent promoters led to the isolation of a gene from Bacillus subtilis, designated spoVR, which appears to be involved in spore cortex formation. Cultures of strains carrying mutations in spoVR had an increased proportion of phase-dark spores, which correlated with an increased proportion of cortexless spores seen by electron microscopy. The numbers of heat- and chloroform-resistant phase-bright spores produced by these mutants were decreased by about 3- to 10-fold, and accumulation of dipicolinate was decreased by more than 3-fold. The spoVR gene was located on the B. subtilis chromosome immediately upstream from, and in the opposite orientation of, the phoAIV gene. Expression of spoVR was initiated at the second hour of sporulation from a sigma E-dependent promoter, and this expression did not require any of the other known mother-cell-specific transcriptional regulators. The spoVR gene was predicted to encode a product of 468 residues.
对依赖σE的启动子进行筛选，导致从枯草芽孢杆菌中分离出一个基因，命名为spoVR，该基因似乎参与芽孢皮层的形成。携带spoVR突变的菌株培养物中，暗相芽孢的比例增加，这与电子显微镜观察到的无皮层芽孢比例增加相关。这些突变体产生的耐热和耐氯仿的亮相芽孢数量减少了约3至10倍，吡啶二羧酸的积累减少了3倍以上。spoVR基因位于枯草芽孢杆菌染色体上，紧挨着phoAIV基因的上游，且方向相反。spoVR的表达在芽孢形成的第二小时从一个依赖σE的启动子开始，这种表达不需要任何其他已知的母细胞特异性转录调节因子。spoVR基因预计编码一个由468个残基组成的产物。
