Orevi N, Falk R
Mutat Res. 1975 Dec;33(2-3):193-200. doi: 10.1016/0027-5107(75)90195-5.
Temperature-sensitive mutations, at the prune locus (pnts) of Drosophilia melanogaster, were induced with EMS. The majority of the new class of mutations, designated pnts-e, do not interact with the mutation Killer of prune (Kpn) at all of the experimental temperatures, i.e., pnts-e, Kpn flies do not die. One mutation, designated pnts-ek, is temperature-senstive with respect to both the eye coloration and the interaction with Kpn. pntr (temperature-non-sensitive), pnts-e, and pnts-ek alleles were found to differ with respect to both quantity of drosopterines and the color of the eyes. The temperature-sensitive period (TSP) for pn eye color occurs at a late pupal stage. The TSP for the pn--Kpn interaction lasts from the beginning of the first laraval instar to eclosion [22]. It is concluded that all pn mutations known to date occupy the same functional unit. The various pn mutations affect the same metabolic step to various degrees: the eye color phenotype results from a reduction in the end product, while the interaction with Kpn depends on the amount of the accumulated precursor. No recombination between pn alleles was found among more than 106 zygotes that were scored. The reasons for this failure are discussed.
利用甲基磺酸乙酯（EMS）诱导黑腹果蝇（Drosophila melanogaster）的修剪基因座（pnts）产生温度敏感突变。大多数新的突变类型，即指定为pnts - e的突变，在所有实验温度下都不与修剪基因杀手（Kpn）突变相互作用，也就是说，pnts - e、Kpn果蝇不会死亡。有一个突变，指定为pnts - ek，在眼睛着色以及与Kpn的相互作用方面对温度敏感。发现pntr（温度不敏感）、pnts - e和pnts - ek等位基因在果蝇蝶呤的数量和眼睛颜色方面存在差异。pn眼睛颜色的温度敏感期（TSP）出现在蛹后期。pn - Kpn相互作用的TSP从第一龄幼虫开始到羽化持续[22]。得出的结论是，迄今为止已知的所有pn突变都占据相同的功能单元。各种pn突变在不同程度上影响相同的代谢步骤：眼睛颜色表型是由于终产物减少所致，而与Kpn的相互作用则取决于积累的前体的量。在超过106个被计分的合子中未发现pn等位基因之间的重组。讨论了这种未发生重组的原因。
