Taoka M, Isobe T, Okuyama T, Watanabe M, Kondo H, Yamakawa Y, Ozawa F, Hishinuma F, Kubota M, Minegishi A
Department of Neuroscience, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
J Biol Chem. 1994 Apr 1;269(13):9946-51.
We cloned cDNAs of a novel protein (designated V-1) that has been identified from among the developmentally regulated proteins in the rat cerebellum. Protein sequencing analysis (Taoka, M., Yamakuni, T., Song, S.-Y., Yamakawa, Y., Seta, K., Okuyama, T., and Isobe, T. (1992) Eur. J. Biochem. 207, 615-620) and cDNA sequence analysis revealed that the V-1 protein consists of 117 amino acids and contains 2.5 contiguous repeats of the cdc10/SWI6 motif, which was originally found in the products of the cell cycle control genes of yeasts and the cell fate determination genes in Drosophila and Caenorhabditis elegans. In situ hybridization histochemistry revealed that the expression of the V-1 gene is transiently increased in postmigratory granule cells during postnatal rat cerebellar development and thereafter is markedly suppressed, whereas Purkinje cells constitutively express V-1 mRNA. In contrast, cerebellar granule cells of the staggerer mutant mouse continue to express the V-1 gene even when the granule cells of the normal mouse have ceased to express the V-1 gene, suggesting that the expression of the V-1 gene in granule cells is regulated through the interaction with Purkinje cells. On the basis of these results, we postulate that the V-1 protein has a potential role in the differentiation of granule cells.
我们克隆了一种新蛋白质（命名为V-1）的cDNA，该蛋白质是从大鼠小脑中发育调控蛋白中鉴定出来的。蛋白质测序分析（Taoka, M., Yamakuni, T., Song, S.-Y., Yamakawa, Y., Seta, K., Okuyama, T., and Isobe, T. (1992) Eur. J. Biochem. 207, 615 - 620）和cDNA序列分析表明，V-1蛋白由117个氨基酸组成，包含2.5个连续的cdc10/SWI6基序重复序列，该基序最初在酵母细胞周期控制基因产物以及果蝇和秀丽隐杆线虫的细胞命运决定基因中发现。原位杂交组织化学显示，在出生后大鼠小脑发育过程中，迁移后的颗粒细胞中V-1基因的表达短暂增加，随后显著受到抑制，而浦肯野细胞持续表达V-1 mRNA。相比之下，即使正常小鼠的颗粒细胞已经停止表达V-1基因，蹒跚突变小鼠的小脑颗粒细胞仍继续表达V-1基因，这表明颗粒细胞中V-1基因的表达是通过与浦肯野细胞的相互作用来调控的。基于这些结果，我们推测V-1蛋白在颗粒细胞的分化中具有潜在作用。
