豚鼠心室肌细胞中心脏钠钙交换体的周转率
Turnover rate of the cardiac Na(+)-Ca2+ exchanger in guinea-pig ventricular myocytes.
作者信息
Powell T, Noma A, Shioya T, Kozlowski R Z
机构信息
University Laboratory of Physiology, Oxford.
出版信息
J Physiol. 1993 Dec;472:45-53. doi: 10.1113/jphysiol.1993.sp019935.
Abstract
- Single guinea-pig ventricular myocytes were voltage clamped using the whole-cell configuration of the patch-clamp technique and membrane current generated by the Na(+)-Ca2+ exchange mechanism recorded. 2. Rapid increases in cytosolic free calcium ([Ca2+]i) evoked by flash photolysis of either nitr-5 or DM-nitrophen resulted in current relaxations, arising from a redistribution of exchanger carrier conformations induced by the changes in [Ca2+]i. 3. Relaxation time constants were temperature dependent with a temperature coefficient over a 10 degrees C range (Q10) of approximately 3 and also voltage dependent, decreasing on hyperpolarization for membrane potentials in the range +40 to -80 mV. 4. The experimental results are consistent with consecutive exchange models having electrogenic Na+ translocation steps, together with a site density and turnover rate similar to that for the Na(+)-K+ pump.
摘要
- 使用膜片钳技术的全细胞模式对单个豚鼠心室肌细胞进行电压钳制，并记录由钠钙交换机制产生的膜电流。2. 由硝普钠或二甲硝苯酚的闪光光解引起的胞质游离钙（[Ca2+]i）的快速增加导致电流弛豫，这是由[Ca2+]i变化引起的交换体载体构象重新分布所致。3. 弛豫时间常数与温度有关，在10℃范围内的温度系数（Q10）约为3，并且也与电压有关，对于+40至 -80 mV范围内的膜电位，超极化时减小。4. 实验结果与具有电致钠转运步骤的连续交换模型一致，其位点密度和周转率与钠钾泵相似。
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