Pritchard J H, Ackerman M, Tubis M, Blahd W H
Proc Soc Exp Biol Med. 1976 Feb;151(2):297-302. doi: 10.3181/00379727-151-39196.
An alternative method to radioiodination is outlined for labeling antibodies with a radioactive marker. The method requires the conjugation of the antibody molecule to chelating agents that contain a free amino group and are capable of binding heavy metal ions. Glutaraldehyde is the coupling agent used to bridge the free amino groups on both the chelate and the antibody molecules. 111In is then added to the antibody-chelate conjugate and is bound instantaneously by the chelating portion. Electrophoretic, autoradiographic, immunodiffusion, and hemagglutination techniques were used to confirm the integrity of the 111In-labeled antibodies.
本文概述了一种用于用放射性标记物标记抗体的放射性碘化替代方法。该方法要求将抗体分子与含有游离氨基且能够结合重金属离子的螯合剂偶联。戊二醛是用于连接螯合剂和抗体分子上的游离氨基的偶联剂。然后将铟 - 111添加到抗体 - 螯合物共轭物中，并通过螯合部分立即结合。使用电泳、放射自显影、免疫扩散和血凝技术来确认铟 - 111标记抗体的完整性。
