Batalova T N, Chernokhvostova E V, German G P, Andreeva N A, Fuzailova F N
Zh Mikrobiol Epidemiol Immunobiol. 1975 Jul(7):41-6.
Light chain types of paraproteins G (76), A (28) and D (5), monoclonal macroglobulins (14) and Bence-Jones proteins (11) were determined by immunoelectrophoretic analysis using monospecific antisera to surface (accessible) and nonaccessible determinants of light chains. The types of the G and A paraproteins were determined in the whole sera; D paraproteins and monoclonal macroglobulins were purified before typing. No precipitation occurred with antisera to light chains in 6 out of 28 A paraproteins. In these cases the inhibition of the IgG precipitation with either the anti-kappa or anti-lambda sera allowed to determine the paraprotein type. The kappa:lambda ratio was established for paraproteins of various classes: 1.7 for paraproteins G, 1.0 for paraproteins A, 2.5 for monoclonal macroglobulins and 0.83 for Bence-Jones proteins. All the D paraproteins investigated were of the lambds-type.
使用针对轻链表面（可及）和不可及决定簇的单特异性抗血清，通过免疫电泳分析确定了副蛋白G（76例）、A（28例）和D（5例）的轻链类型、单克隆巨球蛋白（14例）和本周氏蛋白（11例）。G和A副蛋白的类型在全血清中确定；D副蛋白和单克隆巨球蛋白在分型前进行了纯化。28例A副蛋白中有6例与轻链抗血清不发生沉淀反应。在这些情况下，用抗κ或抗λ血清抑制IgG沉淀可确定副蛋白类型。确定了不同类别副蛋白的κ:λ比值：G副蛋白为1.7，A副蛋白为1.0，单克隆巨球蛋白为2.5，本周氏蛋白为0.83。所有检测的D副蛋白均为λ型。
