猫尾肌的组织化学纤维成分

Histochemical fiber composition of cat's tail muscles.

作者信息

Wada N, Miyata H, Tokuriki M

机构信息

Department of Veterinary Physiology, Faculty of Agriculture, Yamaguchi University, Japan.

出版信息

Arch Ital Biol. 1994 Jan;132(1):53-8.

PMID:8147697

Abstract

A histochemical analysis was performed on the activity of myofibrillar ATPase following preincubation at pH 10.3 with NADH-diaphorase in the cat tail muscles (ECM; extensor caudae medialis, ECL; extensor caudae lateralis, ACE; abductor caudae externus, ACI; abductor caudae internus, FCL; flexor caudae longus, and FCB; flexor caudae brevis). Muscles contained three types of muscle fibers: FG (fast-twitch glycolytic) showed high reaction of myofibrillar ATPase staining and low reaction in NADH-diaphorase staining; FOG (fast-twitch oxidative glycolytic) showed high reaction in myofibrillar ATPase staining and high reaction in NADH-diaphorase staining; and SO (slow-twitch oxidative) showed low reaction in myofibrillar ATPase staining and high reaction in NADH-diaphorase staining. All 6 tail muscles were composed of these three types of fibers, but proportions differed in each tail muscle. Proportions of SO and FG fibers were highest in ECL (SO: 38.6 +/- 2.3, S.D. %) and ACI (FG: 59.2 +/- 5.0%), respectively. The diameters of the fibers were also measured (SO; 50.47 +/- 3.12, FOG; 58.18 +/- 2.78, FG; 70.91 +/- 3.40, S.D. microns).

摘要

对猫尾部肌肉（内侧尾伸肌、外侧尾伸肌、外侧尾展肌、内侧尾展肌、长尾屈肌和短尾屈肌）在pH 10.3下与NADH - 黄递酶预孵育后的肌原纤维ATP酶活性进行了组织化学分析。肌肉包含三种类型的肌纤维：快缩糖酵解型（FG）肌原纤维ATP酶染色反应高，NADH - 黄递酶染色反应低；快缩氧化糖酵解型（FOG）肌原纤维ATP酶染色反应高，NADH - 黄递酶染色反应高；慢缩氧化型（SO）肌原纤维ATP酶染色反应低，NADH - 黄递酶染色反应高。所有6块尾部肌肉均由这三种类型的纤维组成，但各尾部肌肉中纤维比例不同。外侧尾伸肌中慢缩氧化型（SO）和快缩糖酵解型（FG）纤维比例分别最高（SO：38.6±2.3，标准差%）和内侧尾展肌（FG：59.2±5.0%）。还测量了纤维直径（SO；50.47±3.12，FOG；58.18±2.78，FG；70.91±3.40，标准差微米）。