Fukuda N, Yoshihara S, Ichimura T, Yanaihara N
First Department of Pediatrics, Dokkyo University School of Medicine.
Arerugi. 1994 Jan;43(1):55-61.
Chromogranin A like immunoreactivity (CGA-IR) was measured by a specific radioimmunoassay using N-terminal specific antiserum R-0763 in respiratory tissues of guinea pigs. All guinea pig were actively immunized by recurrent Ovalbumin (OA)-inhalation and divided into two groups; one was challenged by OA, and the other inhaled saline as a control. These groups were studied for respiratory resistance and sacrificed for measurement of CGA-IR concentrations in the trachea, major bronchus and lower lung. In the control group, CGA-IR level was 0.4-2 pmol/g wet weight of tissue, and its distribution order was lower lung > trachea > major bronchus. In the OA-challenged group, provocation clearly induced significant elevation of CGA-IR in the trachea and main bronchus coinciding with elevation of respiratory resistance. In the lower lung, on the other hand, the increase in CGA-IR was not significant. These results suggest that experimental immediate asthmatic response (IAR) possibly acts as a stressor to the sympathetic nervous systems in guinea pig air-ways.
使用N端特异性抗血清R-0763，通过特异性放射免疫测定法在豚鼠的呼吸组织中测量嗜铬粒蛋白A样免疫反应性（CGA-IR）。所有豚鼠均通过反复吸入卵清蛋白（OA）进行主动免疫，并分为两组；一组用OA激发，另一组吸入生理盐水作为对照。对这些组进行呼吸阻力研究，并宰杀以测量气管、主支气管和下肺中的CGA-IR浓度。在对照组中，CGA-IR水平为0.4-2 pmol/g组织湿重，其分布顺序为下肺>气管>主支气管。在OA激发组中，激发明显诱导气管和主支气管中CGA-IR显著升高，同时呼吸阻力升高。另一方面，在下肺中，CGA-IR的增加不显著。这些结果表明，实验性速发型哮喘反应（IAR）可能作为一种应激源作用于豚鼠气道中的交感神经系统。
