Fischer T C, Groner S, Zentgraf U, Hemleben V
Lehrstuhl für Allgemeine Genetik, Biologisches Institut, Universität Tübingen, Bundesrepublik Deutschland.
Z Naturforsch C J Biosci. 1994 Jan-Feb;49(1-2):79-86. doi: 10.1515/znc-1994-1-213.
The nucleosomal organization and the protein-binding capability of highly repeated and methylated satellite DNA of cucumber (Cucumis sativus L.), comprising approx. 30% of the genome, were analyzed. Nucleosomal core DNA from satellite type I was prepared after micrococcal nuclease digestion of chromatin and sequenced. Most of the core sequences obtained could be grouped in two main (A and B) and two minor groups (C and D) indicating a specific and complex phasing of nucleosomes on this satellite DNA. In vitro, gel retardation assays with cloned satellite DNA repeats (types I-IV) demonstrated a specific binding of nuclear proteins. These specific binding effects are also obtained with genomic, in vivo methylated and sequence heterogeneous (1 to 10% diversity) satellite type I DNA. For the first time in plants, a satellite DNA-binding protein with an apparent molecular weight of 14 kDa (SAT 14) was identified.
对黄瓜（Cucumis sativus L.）中高度重复且甲基化的卫星DNA的核小体组织和蛋白质结合能力进行了分析，该卫星DNA约占基因组的30%。通过微球菌核酸酶消化染色质制备了来自卫星I型的核小体核心DNA并进行测序。获得的大多数核心序列可分为两个主要组（A和B）和两个次要组（C和D），这表明该卫星DNA上核小体存在特定且复杂的相位。在体外，用克隆的卫星DNA重复序列（I-IV型）进行凝胶阻滞试验，证明了核蛋白的特异性结合。用基因组的、体内甲基化的和序列异质性（1%至10%多样性）的卫星I型DNA也能获得这些特异性结合效应。在植物中首次鉴定出一种表观分子量为14 kDa的卫星DNA结合蛋白（SAT 14）。
