Gaff C, du Sart D, Kalitsis P, Iannello R, Nagy A, Choo K H
Murdoch Institute for Research into Birth Defects, Royal Children's Hospital, Parkville, Victoria, Australia.
Hum Mol Genet. 1994 May;3(5):711-6. doi: 10.1093/hmg/3.5.711.
We have previously reported the identification of a naturally occurring junction between alpha satellite and satellite III DNA on human chromosomes 13, 14 and 21. Direct sequence analysis has shown that the 9 bp alphoid-derived direct repeat sequence (GTGAAAAAG) present at the junction is fully conserved on these chromosomes. A novel protein, pJ alpha, present in HeLa nuclear extracts, binds to the conserved junction sequence. Mutation analysis of the binding site suggests that pJ alpha can recognize one of the two 9 bp repeats and provides some insight into nucleotides that are important for binding. Competition studies support the possibility that this protein binds a significant portion of genomic alpha satellite DNA. Preliminary protein purification experiments have shown that pJ alpha has a molecular weight of 10-15 kDa.
我们之前曾报道过在人类13号、14号和21号染色体上鉴定出α卫星DNA与卫星III DNA之间自然存在的连接区域。直接测序分析表明，位于该连接区域的9个碱基对的α卫星衍生直接重复序列（GTGAAAAAG）在这些染色体上完全保守。一种存在于HeLa细胞核提取物中的新型蛋白质pJα，可与保守的连接序列结合。结合位点的突变分析表明，pJα能够识别两个9碱基对重复序列中的一个，并为对结合重要的核苷酸提供了一些见解。竞争研究支持了这种蛋白质结合基因组中相当一部分α卫星DNA的可能性。初步的蛋白质纯化实验表明，pJα的分子量为10 - 15 kDa。
