Steptoe R J, McMenamin P G, McMenamin C
Department of Anatomy and Human Biology, University of Western Australia, Nedlands.
Br J Ophthalmol. 1994 Mar;78(3):211-8. doi: 10.1136/bjo.78.3.211.
Despite the implication that choroidal mast cells are involved in the onset of experimental autoimmune uveoretinitis (EAU), a widely used animal model of uveoretinitis, little is known of these cells. In the present study the distribution, total number, regional density, and phenotype of choroidal mast cells were examined in Lewis, Wistar Furth, PVG/c, and brown Norway rats. Choroidal mast cells were predominantly associated with arteries and arterioles of more than 30 microns diameter which lie in the outer (sclerad) choroid. The density of mast cells was greatest in the posterior choroid with density diminishing anteriorly. The choroid of male Lewis rats contained significantly greater number of mast cells than that of females (p < 0.01). Histochemical (Alcian blue/safranin) and immunohistochemical (anti-rat mast cell protease I and II monoclonal antibodies) studies revealed choroidal mast cells were of the connective tissue type. However, granule proteinase content appeared less than that of well characterised connective tissue mast cell populations such as those in mesentery and skin. Lewis rats exhibited the highest density of choroidal mast cells (23.6 (SD 1.2)/mm2), Wistar Furth approximately half that of Lewis (13.5 (0.7)/mm2) while PVG/c and brown Norway rats had very low densities (3.06(0.3); 1.95(0.2/mm2 respectively). These studies provide valuable choroidal mast cell data for rats which may have implications for our understanding of experimental models of intraocular inflammation and clinical uveitis.
尽管有迹象表明脉络膜肥大细胞参与了实验性自身免疫性葡萄膜视网膜炎（EAU，一种广泛应用的葡萄膜视网膜炎动物模型）的发病过程，但对这些细胞却知之甚少。在本研究中，我们检测了Lewis、Wistar Furth、PVG/c和棕色挪威大鼠脉络膜肥大细胞的分布、总数、区域密度和表型。脉络膜肥大细胞主要与位于脉络膜外层（巩膜侧）直径超过30微米的动脉和小动脉相关。肥大细胞密度在脉络膜后部最大，向前密度逐渐降低。雄性Lewis大鼠脉络膜中的肥大细胞数量明显多于雌性（p < 0.01）。组织化学（阿尔辛蓝/番红）和免疫组织化学（抗大鼠肥大细胞蛋白酶I和II单克隆抗体）研究显示脉络膜肥大细胞属于结缔组织型。然而，颗粒蛋白酶含量似乎低于肠系膜和皮肤等特征明确的结缔组织肥大细胞群体。Lewis大鼠脉络膜肥大细胞密度最高（23.6（标准差1.2）/mm²），Wistar Furth约为Lewis的一半（13.5（0.7）/mm²），而PVG/c和棕色挪威大鼠的密度非常低（分别为3.06（0.3）；1.95（0.2）/mm²）。这些研究为大鼠提供了有价值的脉络膜肥大细胞数据，这可能对我们理解眼内炎症的实验模型和临床葡萄膜炎有重要意义。
