Pancreatic B-cell culture after intravitam B-cell selection with nitric pilocarpine and modified B-technique use.

Our purpose was intravitam selection of endocrine pancreatic tissue. We decided to use 3% nitric pilocarpine as oral treatment for normal Wistar rats. This experiment was made on 12 male normal Wistar rats, 8 of them receiving 0.025 ml 3% nitric pilocarpine in a single daily dose, for 7 days. In the next 3-4 days, all of these have demonstrated clinical picture of exocrine pancreatic failure leading finally to death. Animals have received ad libitum food-intake. During this period, the study group has demonstrated glycemia values between 65...75 mg/dl vs. 68...85 mg/dl for normal group. The histological picture of study group's pancreatic pieces demonstrated intensive necrobiotic changes for exocrine components, but normal features for the exocrine ones. Furthermore we have obtained primary monolayer cultures of this dominant B-cell tissue, using a modified B-technique. Our results demonstrated the greatest percentage of B-cells on the cultures, in comparison with the non-using pilocarpine method (other studies). The presence of mega-beta-islet cells in primary cultures, as well as the lack of fibroblast population, demonstrated the efficiency of this method.