Turková J, Valentová O, Coupek J
Biochim Biophys Acta. 1976 Feb 20;420(2):309-15. doi: 10.1016/0005-2795(76)90322-6.
A mixture of aminopeptidase and neutral protease from the Aspergillus flavus mold obtained by chromatography on DEAE-Sephadex was fractionated by chromatography on the hydroxyalkyl methacrylate gel with chemically bonded 1,6 hexamethylene diamine and D-leucine. Aminopeptidase thus obtained was electrophoretically homogeneous. Conditions for chromatography were worked out allowing a one stage isolation of a highly active aminopeptidase sample directly from the alcoholic precipitate of the culture medium of the Aspergillus flavus mold.
通过DEAE-葡聚糖凝胶柱层析从黄曲霉霉菌中获得的氨肽酶和中性蛋白酶混合物，再用化学键合有1,6-己二胺和D-亮氨酸的甲基丙烯酸羟烷基酯凝胶进行柱层析分离。由此得到的氨肽酶在电泳上是均一的。确定了层析条件，可直接从黄曲霉霉菌培养基的乙醇沉淀物中一步分离出高活性氨肽酶样品。
