Mannion D, Fitzpatrick G J, Feeley M
Department of Anesthesia and Critical Care, Meath Hospital, Trinity College, Dublin, Ireland.
Circ Shock. 1994 Jan;42(1):39-43.
The irreversible loss of adenine nucleotides and the formation of free radicals have both been suggested as causes of irreversibility following prolonged hemorrhagic shock. This study was performed to assess the effect of xanthine oxidase inhibition (allopurinol 50 mg/kg/day), free radical scavenging (superoxide dismutase 15,000 u/kg, catalase 15,000 u/kg, dimethylsulfoxide 20 mg/kg, and alpha tocopherol 100 mg/kg/day) or both, on the 24-hr survival of dogs subjected to irreversible haemorrhagic shock. Twenty anesthetized dogs were bled to a mean arterial pressure of 30 mm Hg for 4 hr. The dogs were allocated to a control, an allopurinol pretreated, a free radical scavenger, or a combined treatment group. Both groups pretreated with allopurinol had significantly improved survival (P < 0.05) over that seen in the control group, but the free radical scavenger treated group was not significantly different from the control group. This study demonstrates the beneficial effect of xanthine oxidase inhibition on survival, and suggests that it may be due to preservation of adenine nucleotides rather than prevention of free radical formation.
腺嘌呤核苷酸的不可逆损失和自由基的形成均被认为是长时间失血性休克后不可逆性的原因。本研究旨在评估黄嘌呤氧化酶抑制（别嘌呤醇50毫克/千克/天）、自由基清除（超氧化物歧化酶15,000单位/千克、过氧化氢酶15,000单位/千克、二甲亚砜20毫克/千克和α-生育酚100毫克/千克/天）或两者联合使用对遭受不可逆性失血性休克的犬24小时存活率的影响。将20只麻醉的犬放血至平均动脉压为30毫米汞柱并持续4小时。这些犬被分配至对照组、别嘌呤醇预处理组、自由基清除剂组或联合治疗组。与对照组相比，两个用别嘌呤醇预处理的组的存活率均有显著提高（P<0.05），但自由基清除剂治疗组与对照组无显著差异。本研究证明了黄嘌呤氧化酶抑制对存活率的有益作用，并表明这可能是由于腺嘌呤核苷酸的保存而非自由基形成的预防。
