Flett R J, Hamilton R D, Campbell N E
Can J Microbiol. 1976 Jan;22(1):43-51. doi: 10.1139/m76-006.
Previous methods of performing aquatic acetylene-reduction assays are described and several problems associated with them are discussed. A refinement of these older techniques is introduced and problems that it overcomes are also discussed. A depth profile of nitrogen fixation (C2H4 production), obtained by the refined technique, is shown for a fertilized Canadian Shield lake in the Experimental Lakes Area of northwestern Ontario.
本文描述了以往进行水生乙炔还原测定的方法，并讨论了与之相关的几个问题。介绍了对这些旧技术的改进，并讨论了它所克服的问题。通过改进技术获得了安大略省西北部实验湖区一个施肥的加拿大地盾湖的固氮深度剖面（乙烯生成量）。
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