Genco C A, Berish S A, Chen C Y, Morse S, Trees D L
Department of Microbiology and Immunology, Morehouse School of Medicine, Atlanta, Georgia 30310.
FEMS Microbiol Lett. 1994 Feb 15;116(2):123-9. doi: 10.1111/j.1574-6968.1994.tb06690.x.
The pathogenic Neisseria and most commensal Neisseria species produce an iron-binding protein (Fbp) when grown under iron-limited conditions. In the current study, we confirmed the presence of Fbp, as well as DNA sequences homologous to the gonococcal fbp, in strains of N. gonorrhoeae, N. meningitidis, N. cinerea, N. lactamica, N. subflava, N. kochii and N. polysaccharea. The fbp genes from these strains were amplified by the polymerase chain reaction, digested with StuI or RsaI, and the restriction patterns examined. The patterns for the gonococcal and meningococcal fbp were virtually identical; however, variations were observed in the fbp sequences of the commensal Neisseria species. N. flavescens, N. mucosa, N. sicca, N. ovis and Branhamella catarrhalis, did not produce Fbp as detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and reactivity with an Fbp specific monoclonal antibody, nor did they hybridize to an fbp-specific DNA probe.
致病性奈瑟菌和大多数共生奈瑟菌在铁限制条件下生长时会产生一种铁结合蛋白（Fbp）。在本研究中，我们证实了淋病奈瑟菌、脑膜炎奈瑟菌、灰色奈瑟菌、乳糖奈瑟菌、微黄奈瑟菌、 Kochii奈瑟菌和多糖奈瑟菌菌株中存在Fbp以及与淋球菌fbp同源的DNA序列。通过聚合酶链反应扩增这些菌株的fbp基因，用StuI或RsaI进行消化，并检查限制性酶切图谱。淋病奈瑟菌和脑膜炎奈瑟菌fbp的图谱几乎相同；然而，在共生奈瑟菌物种的fbp序列中观察到了差异。微黄奈瑟菌、黏膜奈瑟菌、干燥奈瑟菌、羊奈瑟菌和卡他布兰汉菌，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳检测以及与Fbp特异性单克隆抗体的反应性，均未产生Fbp，它们也未与fbp特异性DNA探针杂交。
