Hu H, de Weger R, Schuurman H J
Department of Pathology, University Hospital, Utrecht, The Netherlands.
Hua Xi Yi Ke Da Xue Xue Bao. 1993 Dec;24(4):435-8.
A method of positive identification of the specificity of polymerase chain reaction (PCR) product using internal oligonucleotide probe is introduced. The hybridization was done on the agarose gel which was dried after electrophoresis. Detection of the expression of T cell receptor a chain variable (TCR V alpha) genes on mRNA level was used as the experimental model. Twenty nine TCR V alpha gene subfamilies could be distinguished clearly in healthy human peripheral blood lymphocytes by this method. Positive identification of PCR product on dried agarose gel by internal oligonucleotide probe is relatively simple and less time consuming.
介绍了一种使用内部寡核苷酸探针阳性鉴定聚合酶链反应（PCR）产物特异性的方法。杂交在电泳后干燥的琼脂糖凝胶上进行。以mRNA水平检测T细胞受体α链可变区（TCR Vα）基因的表达作为实验模型。通过该方法可在健康人外周血淋巴细胞中清晰区分29个TCR Vα基因亚家族。通过内部寡核苷酸探针在干燥琼脂糖凝胶上对PCR产物进行阳性鉴定相对简单且耗时较少。
