Aggarwal S J, Diller K R, Blake G K, Baxter C R
Department of Mechanical Engineering, University of Texas at Austin 78712-1084.
J Burn Care Rehabil. 1994 Jan-Feb;15(1):1-12. doi: 10.1097/00004630-199401000-00002.
Alterations in the cutaneous vasomotion function caused by scald burns to a large area on the flank were observed and quantified in a dorsal skin flap window with a hamster model. Subsequent to chronic implantation of the window chamber to provide direct microscopic observation of blood flow for an entire thickness of skin, control measurements of vasoactivity in a defined network of arterioles were taken by means of a digital video image splitter over a period of several days. A 100 degrees C, 5-second water scald was then effected over 17% to 55% of the total body surface area, and the vasoactivity was remeasured in the targeted set of sites at serial time intervals for 2 to 4 days. Data were acquired directly into a computer and analyzed for both the magnitude and time pattern of diameter fluctuations during 3-minute observation periods. Average and standard deviations of diameters were computed, and the Prony power spectral analysis method was applied to identify the presence and strength of fluctuation frequencies. In general, vasomotion was suppressed for several hours subsequent to the burn, and the activity was dominated by much slower contractions than control. No direction of vessel diameter changes prevailed uniformly throughout the peripheral microcirculation. Large alternate constrictions and dilations were also observed for some arteriolar and venular components, but others exhibited no significant change at all. The incidence and magnitude of peripheral microvascular response was proportional to the size of the injured area.
在仓鼠模型的背侧皮瓣窗口中观察并量化了由侧腹大面积烫伤引起的皮肤血管运动功能改变。在长期植入窗口室以对全层皮肤血流进行直接显微镜观察之后，通过数字视频图像分割器在几天时间内对特定小动脉网络中的血管活性进行对照测量。然后对全身表面积的17%至55%进行100摄氏度、持续5秒的水烫伤，并在2至4天的连续时间间隔内对目标部位的血管活性进行重新测量。数据直接采集到计算机中，并对3分钟观察期内直径波动的幅度和时间模式进行分析。计算直径的平均值和标准差，并应用Prony功率谱分析方法来确定波动频率的存在和强度。一般来说，烧伤后血管运动被抑制数小时，且活动以比对照慢得多的收缩为主导。在整个外周微循环中，血管直径变化的方向并非普遍一致。在一些小动脉和小静脉成分中也观察到大幅度的交替收缩和扩张，但其他一些成分则根本没有明显变化。外周微血管反应的发生率和幅度与受伤面积的大小成正比。
