Marnet P G, Volland H, Pradelles P, Grassi J, Beaufils M
INRA, Laboratoire de Recherches sur la Traite ENSAR, Rennes.
J Immunoassay. 1994 Feb;15(1):35-53. doi: 10.1080/15321819408009570.
The pure tetrameric form of Acetylcholinesterase (EC-3.1.1.7) from the electric eel electrophorus electricus has been covalently coupled to oxytocin. This conjugate has been used as tracer in a heterologous competitive immunoassay. Microtiter plates coated with a mouse monoclonal anti-rabbit immunoglobulin antibody were used to separate bound and free moieties of the tracer. Acetylcholinesterase activity bound to the solid phase was measured by a colorimetric assay. The minimum detectable concentration was 0.075 pg/well (ie 1.5 pg/ml) and precision was less than 8% at concentration above 0.15 pg/well. An extraction step improved sensitivity up to 10 times with good recoveries. To assess the validity of this assay, basal levels of oxytocin were measured during the oestrous cycle of a cow.
将来自电鳗（电美洲鳗）的乙酰胆碱酯酶（EC-3.1.1.7）的纯四聚体形式与催产素共价偶联。这种偶联物已被用作异源竞争免疫测定中的示踪剂。用包被有小鼠单克隆抗兔免疫球蛋白抗体的微量滴定板来分离示踪剂的结合部分和游离部分。通过比色测定法测量与固相结合的乙酰胆碱酯酶活性。最低可检测浓度为0.075 pg/孔（即1.5 pg/ml），在浓度高于0.15 pg/孔时精密度小于8%。一个提取步骤将灵敏度提高了10倍且回收率良好。为评估该测定法的有效性，在一头奶牛的发情周期中测量了催产素的基础水平。
