Bendixen B, Rickwood D
Department of Biology, University of Essex, Colchester, UK.
J Biochem Biophys Methods. 1994 Jan;28(1):43-52. doi: 10.1016/0165-022x(94)90063-9.
This paper reports on the properties of a new iodinated density-gradient medium, Iotrolan, and its potential application for separating dense cells. Iotrolan is a dimeric, nonionic, iodinated compound which is distinguished by its ability to form dense, non-viscous gradients of low osmolarity. Isotonic gradients of up to 1.30 g/ml can be formed, allowing even dense cells such as sperm to be separated under isotonic conditions.
