Schmitt I M, Maxwell V M, Olack G A, Edelson R L, Chimenti S, Gasparro F P
Department of Dermatology, University of L'Aquila, Italy.
J Photochem Photobiol B. 1994 Jan;22(1):17-21. doi: 10.1016/1011-1344(93)06946-z.
The effects of 8-methoxypsoralen (8-MOP) monoadducts and crosslinks on growth and viability of mastocytoma cells were investigated. To induce monoadduct formation (4',5'-monoadducts and 3,4-monoadducts), the cells were incubated with 8-MOP (1 microgram ml-1) and exposed to 419 nm radiation, resulting in the formation of more than 96% monoadducts. After washing and resuspension, the cells were exposed to a small dose of long-wavelength UV radiation (UVA, 2 J cm-2) to convert monoadducts into crosslinks. Similar adduct levels were obtained after either 8-MOP plus visible light treatment or 8-MOP plus split-dose protocol. Cells treated with 419 nm light resumed normal growth rates more rapidly than cells which also received the UVA dose. High performance liquid chromatography (HPLC) analysis of DNA obtained from each group of cells showed that the UVA step resulted in an increase in crosslinks from 3.2% after 419 nm radiation to 56.5% after UVA irradiation.
研究了8-甲氧基补骨脂素（8-MOP）单加合物和交联物对肥大细胞瘤细胞生长和活力的影响。为诱导单加合物形成（4',5'-单加合物和3,4-单加合物），将细胞与8-MOP（1微克/毫升）孵育并暴露于419纳米辐射，形成了超过96%的单加合物。洗涤并重悬后，将细胞暴露于小剂量的长波长紫外线辐射（UVA，2焦耳/平方厘米），以将单加合物转化为交联物。8-MOP加可见光处理或8-MOP加分次给药方案后获得了相似的加合物水平。用419纳米光处理的细胞比也接受UVA剂量的细胞更快恢复正常生长速率。对每组细胞获得的DNA进行高效液相色谱（HPLC）分析表明，UVA步骤导致交联物从419纳米辐射后的3.2%增加到UVA照射后的56.5%。
