一种蛋白酶激活突变体MVCES1，作为一种源自当前流行的仙台病毒的安全且有效的活疫苗。
A protease activation mutant, MVCES1, as a safe and potent live vaccine derived from currently prevailing Sendai virus.
作者信息
Wang X L, Itoh M, Hotta H, Homma M
机构信息
Department of Microbiology, Kobe University School of Medicine, Japan.
出版信息
J Virol. 1994 May;68(5):3369-73. doi: 10.1128/JVI.68.5.3369-3373.1994.
Abstract
Sendai virus fresh isolates were shown to be antigenically different from the prototype Fushimi strain that had long been passaged in embryonated chicken eggs. Phylogenetic analysis of the hemagglutinin-neuraminidase genes also revealed the difference between these two virus groups. Both trypsin-resistant and elastase-sensitive mutations were additionally introduced to an LLC-MK2-cell-adapted and attenuated mutant derived from one of the fresh isolates. This protease activation mutant (MVCES1) showed the same antigenicity as the fresh isolates, and as a result of a single cycle of growth in lungs, it could confer better protection on mice against challenge infection with the currently prevailing Sendai virus than TR-5, which is a trypsin-resistant mutant derived from the Fushimi strain. The eligibility of MVCES1 as an attenuated live vaccine of Sendai virus is discussed.
摘要
研究表明，仙台病毒新鲜分离株在抗原性上与长期在鸡胚中传代的原型伏见株不同。对血凝素 - 神经氨酸酶基因的系统发育分析也揭示了这两组病毒之间的差异。此外，对源自其中一个新鲜分离株的LLC - MK2细胞适应型减毒突变株引入了抗胰蛋白酶和对弹性蛋白酶敏感的突变。这种蛋白酶激活突变株（MVCES1）与新鲜分离株具有相同的抗原性，并且由于在肺部进行单周期生长，与源自伏见株的抗胰蛋白酶突变株TR - 5相比，它能为小鼠提供更好的保护，使其免受当前流行的仙台病毒的攻击感染。本文讨论了MVCES1作为仙台病毒减毒活疫苗的适用性。
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