臭鼩肝脏中药物代谢酶的活性：肝细胞溶胶中可能缺乏N-乙酰转移酶活性。

Activities of drug-metabolizing enzymes in the liver of Suncus murinus: possible lack of N-acetyltransferase activity in liver cytosol.

作者信息

Nakura H, Itoh S, Kusano H, Ishizone H, Kamataki T

机构信息

Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.

出版信息

Pharmacology. 1994 Mar;48(3):201-4. doi: 10.1159/000139180.

DOI:10.1159/000139180

PMID:8153148

Abstract

The amounts of several drug-metabolizing enzymes in livers of Suncus murinus (suncus) were studied in comparison with rats. The content of cytochrome P450 in suncus was less than one third that seen in rats. Activities of glutathione S-transferase, UDP-glucuronyltransferase and arylhydroxycarbon hydroxylase in suncus were less than half those in rats. Activity of flavin-containing monooxygenase in suncus was 71% that of rats. Interestingly, N-acetyltransferase (NAT) activity in suncus liver cytosols was not detectable: no detectable activities were seen with aniline, p-aminobenzoic acid and p-amino-salicylic acid as substrates. We propose that suncus is a unique animal possibly lacking NAT in liver cytosol.

摘要

研究了臭鼩肝脏中几种药物代谢酶的含量，并与大鼠进行了比较。臭鼩细胞色素P450的含量不到大鼠的三分之一。臭鼩中谷胱甘肽S-转移酶、UDP-葡萄糖醛酸基转移酶和芳基羟化碳羟化酶的活性不到大鼠的一半。臭鼩中含黄素单加氧酶的活性为大鼠的71%。有趣的是，在臭鼩肝脏胞质溶胶中未检测到N-乙酰转移酶（NAT）活性：以苯胺、对氨基苯甲酸和对氨基水杨酸为底物时未检测到活性。我们提出臭鼩是一种独特的动物，其肝脏胞质溶胶中可能缺乏NAT。