Du Z F, Wales R G
School of Veterinary Studies, Murdoch University, Australia.
Reprod Fertil Dev. 1993;5(4):395-403. doi: 10.1071/rd9930395.
Samples of trophoblast recovered from the sheep conceptus on Day 13 of pregnancy formed spherical vesicles during culture in medium 199. These continued to expand and increase in dry weight over the next 6 days in vitro. After 6 days' culture, the metabolism of glucose by these vesicles was compared with that of Day-13 and Day-19 fresh trophoblastic tissue. The production of CO2 and lactate by vesicles was similar, although not identical, to production by Day-13 fresh tissue and did not exhibit the marked decrease in glucose catabolism seen in Day-19 trophoblast. The tissue from vesicles reacted to reduction in oxygen tension in a manner similar to the reaction of fresh tissue, with decreased glucose oxidation and increased glycolysis. The activity of the pentose phosphate pathway in vesicles was higher, and the activity in Day-19 fresh tissue was much lower, than that in Day-13 fresh trophoblast. Incorporation of glucose into the intracellular biochemical pools by vesicles was similar to incorporation into Day-13 fresh tissue. Limited observations were also made with vesicles derived from embryonic disc. Production of CO2 by these vesicles was intermediate between that detected in fresh Day-13 and Day-19 embryonic tissue. There were not significant differences in lactate production between fresh and cultured samples of embryonic tissue. These results show that vesicles formed in vitro remain metabolically active but do not mimic the biochemical changes seen in the tissue during development in vivo.
在怀孕第13天从绵羊孕体回收的滋养层样本，在199培养基中培养时形成了球形囊泡。在接下来的6天体外培养中，这些囊泡持续扩张且干重增加。培养6天后，将这些囊泡的葡萄糖代谢与第13天和第19天的新鲜滋养层组织的葡萄糖代谢进行比较。囊泡产生二氧化碳和乳酸的情况与第13天新鲜组织的产生情况相似，虽不完全相同，且未表现出第19天滋养层中所见的葡萄糖分解代谢的显著下降。囊泡组织对氧张力降低的反应方式与新鲜组织的反应方式相似，葡萄糖氧化减少，糖酵解增加。囊泡中磷酸戊糖途径的活性较高，而第19天新鲜组织中的活性远低于第13天新鲜滋养层中的活性。囊泡将葡萄糖掺入细胞内生化池的情况与掺入第13天新鲜组织的情况相似。对源自胚盘的囊泡也进行了有限的观察。这些囊泡产生二氧化碳的情况介于第13天新鲜胚胎组织和第19天新鲜胚胎组织中检测到的情况之间。新鲜胚胎组织样本和培养样本之间乳酸产生没有显著差异。这些结果表明，体外形成的囊泡仍保持代谢活性，但不能模拟体内发育过程中组织中所见的生化变化。
