用源自具核梭杆菌的两种DNA探针研究具核梭杆菌与脆弱拟杆菌之间的相似性。

Similarities between Fusobacterium nucleatum and bacteroides fragilis studied by two DNA probes derived from Fusobacterium nucleatum.

作者信息

Bolstad A I, Kleivdal H, Jensen H B

机构信息

Department of Biochemistry and Molecular Biology, University of Bergen, Norway.

出版信息

Scand J Dent Res. 1994 Feb;102(1):5-9. doi: 10.1111/j.1600-0722.1994.tb01144.x.

DOI:10.1111/j.1600-0722.1994.tb01144.x

PMID:8153579

Abstract

A polymerase chain reaction (PCR)-amplified oligonucleotide DNA probe corresponding to a Fusobacterium nucleatum Fevl DNA region coding for a 40-kDa major outer-membrane protein (OMP) and a randomly cloned 2.1 kb DNA probe were found to recognize DNA from the Gram-negative bacteria Fusobacterium nucleatum and Bacteroides fragilis on Southern blots and slot blots. The results indicate sequence similarity within the DNA fragments studied. Immunoblots tested with polyclonal antibodies against whole cells of F. nucleatum revealed only weak antigen similarity between these species.

摘要

发现一种与具核梭杆菌Fevl DNA区域相对应的聚合酶链反应（PCR）扩增寡核苷酸DNA探针，该区域编码一种40 kDa的主要外膜蛋白（OMP），以及一个随机克隆的2.1 kb DNA探针，在Southern印迹和狭缝印迹上可识别革兰氏阴性菌具核梭杆菌和脆弱拟杆菌的DNA。结果表明在所研究的DNA片段内存在序列相似性。用针对具核梭杆菌全细胞的多克隆抗体进行的免疫印迹显示，这些菌种之间仅存在微弱的抗原相似性。