Nielsen H M, Andreassen T T, Ledet T, Oxlund H
University of Aarhus, Department of Connective Tissue Biology, Denmark.
Acta Orthop Scand. 1994 Feb;65(1):37-41. doi: 10.3109/17453679408993715.
The effect of Transforming Growth Factor beta (TGF-beta) administered locally around the fracture line of healing rat tibial fractures was investigated after 40 days of healing. TGF-beta in a dose of 4 ng or 40 ng was injected every second day during the healing period. The strength, stiffness, energy absorption and deflection of the fractures were measured in a materials-testing machine. Compared with placebo-treated animals, the ultimate load of the fractures increased in the group injected with 40 ng of TGF-beta, but not in those injected with 4 ng. TGF-beta induced a dose-dependent increase in the cross-sectional area of the callus and bone at the fracture line. Consequently, local treatment of fractures with TGF-beta increases the callus formation and strength. The energy absorption and deflection capacities of the healing fractures are preserved.
在大鼠胫骨骨折愈合40天后，研究了在愈合骨折线周围局部给予转化生长因子β（TGF-β）的效果。在愈合期间每隔一天注射4 ng或40 ng剂量的TGF-β。在材料试验机中测量骨折的强度、刚度、能量吸收和挠度。与接受安慰剂治疗的动物相比，注射40 ng TGF-β的组骨折的极限载荷增加，但注射4 ng的组没有增加。TGF-β诱导骨折线处骨痂和骨骼的横截面积呈剂量依赖性增加。因此，用TGF-β局部治疗骨折可增加骨痂形成和强度。愈合骨折的能量吸收和挠曲能力得以保留。
