Friedlaender R P, Grizzard M B, Helms C M, Barile M F, Senterfit L, Chanock R M
J Infect Dis. 1976 Mar;133(3):343-6. doi: 10.1093/infdis/133.3.343.
Knowledge of the pathogenesis of pneumonia due to Mycoplasma pneumoniae has been derived primarily from experimental infection of rodents. As part of an effort to establish a model with a closer resemblance to man, three seronegative, young, adult rhesus monkeys (Macaca mulatta) were inoculated with M. pneumoniae (10(7.4) cfu per animal) by oropharyngeal administration of coarse-particle aerosol. Five to six days after exposure of the animals, cultures obtained from the upper respiratory tract were positive for M. pneumoniae. Each animal subsequently developed a serologic response, as determined by complement fixation, complement-mediated killing, and tetrazolium-reduction inhibition techniques. Infection was subclinical, and serial chest roentgenograms failed to disclose pneumonia throughout the course of infection. Blood cell counts and titers of cold agglutinins remained unchanged. Althought M. pneumoniae was recovered from the upper respiratory tract of two monkeys for 50 days, there was no evidence of transmission of infection to cage-mate controls inoculated with broth.
肺炎支原体所致肺炎发病机制的相关知识主要源于对啮齿动物的实验性感染。作为建立更接近人类模型的努力的一部分，通过口咽给予粗颗粒气溶胶，对三只血清阴性的成年恒河猴（猕猴）接种肺炎支原体（每只动物10(7.4) cfu）。动物暴露五到六天后，从上呼吸道获得的培养物肺炎支原体呈阳性。通过补体结合、补体介导的杀伤和四氮唑还原抑制技术测定，每只动物随后都出现了血清学反应。感染为亚临床型，在整个感染过程中，连续胸部X线片未显示肺炎。血细胞计数和冷凝集素滴度保持不变。虽然在两只猴子的上呼吸道中分离到肺炎支原体达50天，但没有证据表明感染传播给接种肉汤的同笼对照动物。
