植物组织培养抗原的多重复制品及免疫化学交叉反应性

Multiliter production and immunochemical cross-reactivity of plant tissue culture antigens.

作者信息

Wu W L, Staba E J, Blumenthal M N

出版信息

J Pharm Sci. 1976 Jan;65(1):102-5. doi: 10.1002/jps.2600650122.

Abstract

Cells of short ragweed suspension culture were successfully propagated in a multiliter fermentor. Proteins were isolated from tissue cultures (short ragweed, cantaloupe, marigold, and Ammi) and plants (short ragweed pollen, cantaloupe fruit, and marigold flowers) by ammonium sulfate precipitation, cross-linked dextran gel filtration, and anion-exchange chromatography. Some fractions elicited allergenic responses in ragweed-sensitive patients but not in ragweed-nonsensitive control patients. Double-immunodiffusion analysis of these fractions showed their nonidentity with short ragweed antigen E.

摘要

豚草悬浮培养细胞成功地在多升发酵罐中繁殖。通过硫酸铵沉淀、交联葡聚糖凝胶过滤和阴离子交换色谱法从组织培养物（豚草、哈密瓜、万寿菊和阿米芹）和植物（豚草花粉、哈密瓜果实和万寿菊花）中分离蛋白质。一些组分在对豚草敏感的患者中引发了过敏反应，但在对豚草不敏感的对照患者中未引发。对这些组分的双向免疫扩散分析表明它们与豚草抗原E不同。