Björk J, Backman L
Department of Biochemistry, University of Umeå, Sweden.
Biochim Biophys Acta. 1994 Apr 20;1191(1):141-6. doi: 10.1016/0005-2736(94)90241-0.
Addition of sodium selenite to human red cells, under ATP deplete conditions, induces a rapid oxidation of both glutathione and protein sulphydryl groups. Selenite also inhibits the discocyte-echinocyte shape transformation and stops the process before completion. Parallel to the effect on shape, selenite reduces the dephosphorylation of phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-monophosphate. Therefore our results support a shape change mechanism based on the metabolism of phosphoinositides and compatible with the bilayer-couple hypothesis.
在ATP耗尽的条件下，向人红细胞中添加亚硒酸钠会导致谷胱甘肽和蛋白质巯基迅速氧化。亚硒酸钠还会抑制盘状红细胞向棘状红细胞的形态转变，并在转变完成前使其停止。与对形态的影响同时发生的是，亚硒酸钠会减少磷脂酰肌醇4,5-二磷酸和磷脂酰肌醇4-单磷酸的去磷酸化。因此，我们的结果支持一种基于磷酸肌醇代谢且与双层偶联假说相符的形态变化机制。
