Pal'mina N P, Mal'tseva E L, Kurnakova N V, Burlakova E B
Biokhimiia. 1994 Feb;59(2):193-200.
The effect of the natural antioxidant alpha-tocopherol (alpha-TL) on protein kinase C (PK-C) from rabbit heart has been studied. alpha-TL inhibits the PK-C activity; the concentration dependence curve has two maxima-at (10(-4)-10(-6) M) and at (10(-11)-10(-15) M). There is a "zone of silence" between these two regions. The shift of the first maximum towards higher alpha-TL concentrations (5 x 10(-3) M) and the decrease in the values of the both inhibition maxima (from 80-90% to 40-60%) was observed for the PK-C preactivated by 12-O-tetradecanoyl-phorbol-13-acetate. The putative role of alpha-TL and lipid hydroperoxides in the regulation of proliferation of normal and tumour cells is discussed.
研究了天然抗氧化剂α-生育酚（α-TL）对兔心脏蛋白激酶C（PK-C）的作用。α-TL抑制PK-C活性；浓度依赖性曲线有两个最大值——在（10^-4 - 10^-6 M）和（10^-11 - 10^-15 M）。这两个区域之间存在一个“沉默区”。对于经12-O-十四烷酰佛波醇-13-乙酸酯预激活的PK-C，观察到第一个最大值向更高α-TL浓度（5×10^-3 M）的移动以及两个抑制最大值的值的降低（从80 - 90%降至40 - 60%）。讨论了α-TL和脂质氢过氧化物在正常细胞和肿瘤细胞增殖调节中的假定作用。
