Juan C, Pons J, Petitpierre E
Departament de Biologia Ambiental, Universitat de les Illes Balears, Palma de Mallorca, Spain.
Chromosome Res. 1993 Sep;1(3):167-74. doi: 10.1007/BF00710770.
In situ hybridization to chromosomes and nuclei of Tenebrio molitor shows the massive presence of a species-specific satellite DNA in all chromosomes and six sites of rDNA in mitotic chromosomes. These sites are located in two autosomal pairs and in the X and Y chromosomes. In a related species, Misolampus goudoti, in which two different families of highly repetitive DNA have been previously characterized, one family is located in centromeric regions of all chromosomes with the exception of chromosome Y, while the other repeated DNA family is present both in centromeric and distal regions of all chromosomes. rRNA genes in this species are present in a medium-sized autosomal pair only. These results show that molecular cytogenetics can be applied to coleopteran chromosomes and open the way for a physical mapping of DNA sequences in these organisms. The results also provide insights into the type of meiotic association of the X and Y chromosomes in Coleoptera and the distribution of repeated DNAs within the genome of these insects.
对黄粉虫染色体和细胞核进行原位杂交显示，在所有染色体中均大量存在物种特异性卫星DNA，并且在有丝分裂染色体中有六个核糖体DNA位点。这些位点位于两对常染色体以及X和Y染色体上。在一个相关物种——古氏米索甲螨中，先前已鉴定出两个不同的高度重复DNA家族，其中一个家族位于除Y染色体外的所有染色体的着丝粒区域，而另一个重复DNA家族则存在于所有染色体的着丝粒和远端区域。该物种的核糖体RNA基因仅存在于一对中等大小的常染色体中。这些结果表明，分子细胞遗传学可应用于鞘翅目昆虫染色体，并为这些生物体中DNA序列的物理图谱绘制开辟了道路。这些结果还为鞘翅目昆虫X和Y染色体的减数分裂联会类型以及这些昆虫基因组内重复DNA的分布提供了见解。
