Gonzalez A M, Logan A, Ying W, Lappi D A, Berry M, Baird A
Department of Molecular and Cellular Growth Biology, Whittier Institute for Diabetes and Endocrinology, La Jolla, California 92037.
Endocrinology. 1994 May;134(5):2289-97. doi: 10.1210/endo.134.5.8156932.
In situ hybridization and immunohistochemistry were used to map gene expression and protein distribution of basic fibroblast growth factor (FGF-2) in the hypothalamic-pituitary system. Although the expression of FGF-2 mRNA in the pituitary is low, the protein is widely distributed in both its neural and anterior lobes. In the anterior lobe, immunoreactive (ir-) FGF-2 localizes to basement membranes and select endocrine cells. In the neural lobe, ir-FGF-2 is detected in basement membranes, pituicytes, and Herring bodies. Analyses of FGF high affinity receptor (FGFR) immunoreactivity in the anterior pituitary establishes a distribution of FGFR similar to that of FGF-2. In the neural lobe, ir-FGFR is associated with nerve fibers, pituicytes, and Herring bodies. Unlike FGF-2, the distribution of FGFR1 mRNA correlates well with the presence of the immunoreactive receptor. In the hypothalamus, magnocellular neurons of paraventricular and supraoptic nuclei contain ir-FGF-2 and ir-FGFR. In the median eminence, ir-FGF-2 and ir-FGFR is associated with fibers, glial, and endothelial cells. Ependymal and subependymal cells lining the third ventricle also show high levels of ir-FGF-2 and ir-FGFR and mRNAs. Overall, there is a specific and selective distribution of FGF-2 and its high affinity receptor(s) in the hypothalamo-pituitary axis. This localization lead us to postulate a role in neurohypophyseal functions, possibly water balance.
采用原位杂交和免疫组织化学方法，对下丘脑 - 垂体系统中碱性成纤维细胞生长因子（FGF - 2）的基因表达和蛋白质分布进行定位。虽然垂体中FGF - 2 mRNA的表达较低，但该蛋白在神经叶和前叶中均广泛分布。在前叶中，免疫反应性（ir - ）FGF - 2定位于基底膜和特定的内分泌细胞。在神经叶中，在基底膜、垂体细胞和赫林体中检测到ir - FGF - 2。对垂体前叶中FGF高亲和力受体（FGFR）免疫反应性的分析表明，FGFR的分布与FGF - 2相似。在神经叶中，ir - FGFR与神经纤维、垂体细胞和赫林体相关。与FGF - 2不同，FGFR1 mRNA的分布与免疫反应性受体的存在密切相关。在下丘脑中，室旁核和视上核的大细胞神经元含有ir - FGF - 2和ir - FGFR。在正中隆起，ir - FGF - 2和ir - FGFR与纤维、神经胶质细胞和内皮细胞相关。第三脑室衬里的室管膜细胞和室管膜下细胞也显示出高水平的ir - FGF - 2、ir - FGFR和mRNA。总体而言，FGF - 2及其高亲和力受体在下丘脑 - 垂体轴中存在特异性和选择性分布。这种定位使我们推测其在神经垂体功能中发挥作用，可能与水平衡有关。
