Mellert W, Kühborth B, Gembardt C, Munk R
Department of Toxicology of BASF Aktiengesellschaft, Ludwigshaften, Germany.
Food Chem Toxicol. 1994 Mar;32(3):233-7. doi: 10.1016/0278-6915(94)90195-3.
A 2 yr carcinogenicity study of 2-ethylhexyl acrylate (2-EHA) was conducted by applying 25 microliters 21.5, 43 or 85% 2-EHA or 0.015% benzo[a]pyrene (B[a]P) in acetone, three times/wk, to the clipped dorsal skin of male NMRI mice (80 per group). A further group received acetone and served as the vehicle control. After about 7 months of treatment, half of each group was rested from treatment for a period of 2 months, then treated with the promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) for 20 wk followed by observation until termination of the study. The other half of each group received continuous treatment with 2-EHA, B[a]P or acetone, respectively, for 2 yr. Signs signs of skin irritation were apparent in all groups treated with 2-EHA [hyperkeratosis, hyperplasia (acanthosis), crust formation and ulceration]. In the group treated with B[a]P alone or B[a]P with TPA, 79% and 67% of the mice, respectively, bore squamous cell carcinomas. None of the mice treated with acetone or 2-EHA alone developed a skin tumour at the application site. One squamous cell papilloma occurred in each of the groups treated with 2-EHA and TPA, an incidence matched by the single squamous cell papilloma in an untreated area of an acetone control mouse. Thus, 2-EHA proved not to be carcinogenic in the skin of male NMRI mice by epicutaneous administration.
对2-乙基己基丙烯酸酯（2-EHA）进行了一项为期2年的致癌性研究，将25微升21.5%、43%或85%的2-EHA或0.015%的苯并[a]芘（B[a]P）溶于丙酮中，每周3次涂抹于雄性NMRI小鼠（每组80只）剪毛的背部皮肤。另一组接受丙酮作为溶剂对照。治疗约7个月后，每组一半小鼠停止治疗2个月，然后用促癌剂12-O-十四酰佛波醇-13-乙酸酯（TPA）治疗20周，随后观察直至研究结束。每组另一半小鼠分别接受2-EHA、B[a]P或丙酮连续治疗2年。在用2-EHA治疗的所有组中均出现皮肤刺激迹象[角化过度、增生（棘皮症）、结痂形成和溃疡]。在单独用B[a]P或B[a]P与TPA治疗的组中，分别有79%和67%的小鼠发生鳞状细胞癌。单独用丙酮或2-EHA治疗的小鼠在涂抹部位均未发生皮肤肿瘤。在用2-EHA和TPA治疗的组中各出现1例鳞状细胞乳头状瘤，其发生率与1只丙酮对照小鼠未治疗部位出现的1例鳞状细胞乳头状瘤相同。因此，经皮给药证明2-EHA对雄性NMRI小鼠皮肤无致癌性。
