Lavoie J M, Bolduc L, Hélie R, Bergeron R, Lafond S, Cardin S, Trabelsi F, Yamaguchi N
Département d'éducation physique, Université de Montréal, Québec, Canada.
Int J Sports Med. 1994 Feb;15(2):64-9. doi: 10.1055/s-2007-1021021.
The purpose of the present study was to assess the effects of 2-mercaptoacetate (MA), an inhibitor of hepatic fatty acid oxidation, on the metabolic and pancreatic hormone response to a prolonged (3 h) swimming exercise. All rats were first adrenodemedullated and were either submitted for 3 weeks to a normal (5% fat) or a medium-fat diet (MF; 18% fat). After being submitted to an exercise swimming habituation programme for one week, rats under both dietary conditions were either injected with a bolus dose of MA (600 mumol/kg; ip) or with a saline solution. MA and saline injected rats were either sacrificed after a 3-h swimming exercise or after a 3-h resting period. Administration of MA was associated with a lower level of beta-hydroxybutyrate after exercise in rats fed the MF diet, higher resting and exercising blood glucose levels in rats fed the MF diet, and higher resting and exercising levels of hepatic glycogen in rats fed a normal diet. There were, however, no significant effects of MA on free fatty acid, insulin, glucagon, epinephrine, and norepinephrine concentrations in both dietary conditions either at rest or after exercise. Therefore, the present data do not provide any evidence that the administration of MA, an inhibitor of hepatic fatty acid oxidation, influences the pancreatic hormonal response to exercise. There was also no evidence of a lowering effect of MA on blood glucose levels during exercise.
本研究的目的是评估肝脂肪酸氧化抑制剂巯基乙酸盐（MA）对长时间（3小时）游泳运动的代谢及胰腺激素反应的影响。所有大鼠均先进行肾上腺髓质切除，然后分别给予正常（5%脂肪）或中脂肪饮食（MF；18%脂肪）3周。在进行为期一周的游泳运动适应训练后，两种饮食条件下的大鼠分别注射一剂MA（600 μmol/kg；腹腔注射）或生理盐水。注射MA和生理盐水的大鼠在进行3小时游泳运动或3小时休息后处死。在MF饮食喂养的大鼠中，运动后给予MA与较低水平的β-羟基丁酸有关，在MF饮食喂养的大鼠中，静息和运动时血糖水平较高，在正常饮食喂养的大鼠中，静息和运动时肝糖原水平较高。然而，在两种饮食条件下，无论静息还是运动后，MA对游离脂肪酸、胰岛素、胰高血糖素、肾上腺素和去甲肾上腺素浓度均无显著影响。因此，目前的数据没有提供任何证据表明给予肝脂肪酸氧化抑制剂MA会影响胰腺对运动的激素反应。也没有证据表明MA对运动期间的血糖水平有降低作用。
