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[光合作用能量转化过程中初级过程量子产率的测定技术。III. 不同光合生物的实验数据]

[Technique for the determination of the quantum yield of the primary process in the course of photosynthesis energy transformation. III. Experimental data obtained for different photosynthetic organisms].

作者信息

Barskiĭ E L, Borisov A Iu, Il'ina M D, Samuilov V D, Fetisova Z G

出版信息

Mol Biol (Mosk). 1975 Mar-Apr;9(2):275-82.

PMID:815784
Abstract

A relative method in two modifications has been developed for determination of the quantum yield of primary photosynthesis energy conversion. The purple bacteria E. chaposhnikovii and Chr. minutissimum, R. rubrum chromatophores, cell extracts of E. shaposhnikovii, Rps. spheroides strains GA and R-26, pigment-protein complexes from green bacterium Chl. limicola and light subchloroplast particles, enriched in the photosystem I, were investigated. The quantum yields for all these objects were shown to be 90-98%. The accuracy of the relative method used is very high (within 2--4+). The photosynthetic units were proved to be of a multicentral type for all these systems. The quantum yield values permit estimation of the coefficient of efficiency of the primary conversion process as being approximately equal to 35-38% for bacteria and approximately equal to 50% plants.

摘要

已经开发出一种有两种改进形式的相对方法来测定初级光合作用能量转换的量子产率。对紫色细菌查波什尼科夫埃氏菌和极小色杆菌、深红红螺菌的载色体、查波什尼科夫埃氏菌的细胞提取物、球形红假单胞菌GA和R - 26菌株、绿色细菌嗜盐绿菌的色素 - 蛋白复合物以及富含光系统I的轻亚叶绿体颗粒进行了研究。所有这些对象的量子产率显示为90 - 98%。所使用的相对方法的准确度非常高(在2 - 4%以内)。已证明所有这些系统的光合单位都是多中心类型。量子产率值允许估计初级转换过程的效率系数,细菌约为35 - 38%,植物约为50%。

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