Verdaguer N, Mateu M G, Bravo J, Tormo J, Giralt E, Andreu D, Domingo E, Fita I
Departamento de Ingeniería Química, E.T.S.E.I.B., Universidad Politécnica de Cataluña, Barcelona, Spain.
Proteins. 1994 Feb;18(2):201-3. doi: 10.1002/prot.340180212.
The Fab fragment of the neutralizing monoclonal antibody SD6 elicited against foot-and-mouth disease virus (FMDV) C-S8c1 and its complex with a peptide, corresponding to the major antigenic site of FMDV (VP1 residues 136-150, YTASARGDLAHLTTT), have been crystallized using the hanging drop vapor diffusion techniques. For the isolated Fab, crystals diffracting to 2.5 A resolution were obtained at room temperature using ammonium sulfate as precipitant. These crystals are monoclinic, space group C2, and unit cell parameters a = 109.53 A, b = 89.12 A, c = 64.04 A, and beta = 112.9 degrees and contain one Fab molecule per asymmetric unit. Crystals from the complex diffract, at least, to 2.8 A resolution and were obtained, at room temperature, using PEG as precipitant. These crystals are monoclinic, space group P2, and unit cell parameters a = 56.11 A, b = 60.67 A, c = 143.45 A, and beta = 95.4 degrees. Density packing considerations indicate that there are two Fab molecules in the asymmetric unit.
针对口蹄疫病毒（FMDV）C-S8c1产生的中和单克隆抗体SD6的Fab片段及其与对应于FMDV主要抗原位点（VP1第136-150位氨基酸，YTASARGDLAHLTTT）的肽的复合物，已采用悬滴气相扩散技术进行了结晶。对于分离的Fab，在室温下使用硫酸铵作为沉淀剂获得了衍射分辨率为2.5 Å的晶体。这些晶体为单斜晶系，空间群为C2，晶胞参数a = 109.53 Å，b = 89.12 Å，c = 64.04 Å，β = 112.9°，每个不对称单元包含一个Fab分子。复合物的晶体至少衍射到2.8 Å分辨率，在室温下使用聚乙二醇作为沉淀剂获得。这些晶体为单斜晶系，空间群为P2，晶胞参数a = 56.11 Å，b = 60.67 Å，c = 143.45 Å，β = 95.4°。密度堆积考虑表明不对称单元中有两个Fab分子。
