Ban N, Escobar C, Day J, Greenwood A, McPherson A
Department of Biochemistry, University of California at Riverside 92521.
J Mol Biol. 1993 Dec 5;234(3):894-6. doi: 10.1006/jmbi.1993.1637.
A complex between an Fab fragment of an E2 specific feline infectious peritonitis virus neutralizing antibody 730.1.4 and Fab fragment from anti-idiotypic monoclonal antibody 409.5.3, was crystallized from ammonium sulfate using vapor diffusion methods. The complex crystals diffract to about 2.9 A resolution and are of orthorhombic space group P2(1)22(1) with a = 75.2 A, b = 80.6 A and c = 187.6 A. There are two Fab molecules, or one idiotype-anti-idiotype complex, comprising the asymmetric unit. The long 187.6 A c axis suggests that the long axis of the complex might lie along this direction. Although small and radiation-sensitive, crystals are suitable for three-dimensional structural analysis.
利用气相扩散法从硫酸铵中结晶出E2特异性猫传染性腹膜炎病毒中和抗体730.1.4的Fab片段与抗独特型单克隆抗体409.5.3的Fab片段之间的复合物。该复合物晶体的衍射分辨率约为2.9 Å，属于正交空间群P2(1)22(1)，a = 75.2 Å，b = 80.6 Å，c = 187.6 Å。不对称单元包含两个Fab分子，即一个独特型-抗独特型复合物。187.6 Å的长c轴表明复合物的长轴可能沿此方向。尽管晶体较小且对辐射敏感，但仍适用于三维结构分析。
