The effect of homogenisation on the level of scrapie activity detected in cells from spleens of scrapie affected mice.

Suspensions of cells from the spleens of mice inoculated with scrapie agent were titrated for scrapie activity at two stages, before and after disruption to release activity. Similarity between the titres obtained from the two types of suspension provided evidence to support the suggestion that there is one unit of activity per affected cell and that multiplication of scrapie agent is associated with cell replication.