集胞藻PCC 6803含有一个编码色氨酸合酶β亚基的基因，该基因与拟南芥和玉米（Zea mays L.）的trpB基因具有高度同源性。
Synechocystis PCC 6803 contains a single gene for the beta subunit of tryptophan synthase with strong homology to the trpB genes of Arabidopsis and maize (Zea mays L.).
作者信息
Zhao G P, Somerville R L, Chitnis P R
机构信息
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907-1153.
出版信息
Plant Physiol. 1994 Feb;104(2):461-6. doi: 10.1104/pp.104.2.461.
Abstract
We report the sequence of the trpB gene of the cyanobacterium Synechocystis sp. PCC 6803. This gene was cloned from a plasmid library by functional complementation of a trpB mutant of Escherichia coli K-12. Among the known trpB sequences, the Synechocystis gene bears the greatest homology to the duplicated trpB genes of Arabidopsis thaliana and Zea mays. Southern and northern blotting analyses suggest that Synechocystis contains only a single trpB gene. In contrast to all other prokaryotes, Synechocystis has a trpB gene that is monocistronic. Attempts to construct a trpB null mutant of Synechocystis by standard techniques were unsuccessful, suggesting that this organism is unable to concentrate tryptophan from the external medium.
摘要
我们报道了集胞藻6803（Synechocystis sp. PCC 6803）的色氨酸合成酶B（trpB）基因序列。该基因是通过对大肠杆菌K-12的trpB突变体进行功能互补，从质粒文库中克隆得到的。在已知的trpB序列中，集胞藻的该基因与拟南芥和玉米中重复的trpB基因具有最高的同源性。Southern杂交和Northern杂交分析表明，集胞藻仅含有一个trpB基因。与所有其他原核生物不同，集胞藻的trpB基因是单顺反子的。采用标准技术构建集胞藻trpB基因缺失突变体的尝试未成功，这表明该生物体无法从外部培养基中浓缩色氨酸。
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