Busca A, Miniero R, Vassallo E, Leone L, Oddenino O, Madon E
Department of Pediatrics, University of Turin, Italy.
Ther Drug Monit. 1994 Feb;16(1):71-4. doi: 10.1097/00007691-199402000-00012.
In order to assess the suspected influence of indwelling lines on the measurement of cyclosporine (CSA) blood levels, we evaluated CSA concentrations in samples drawn simultaneously from both silicone catheter lumens and peripheral veins of eight bone marrow transplant recipients. Blood samples were obtained at 30 min or 6-12 h after discontinuation of intravenous CSA. CSA concentrations were determined in whole blood by specific monoclonal antibody assay. Mean CSA levels from the lumen used for CSA administration were significantly higher than those from samples drawn from the second lumen (i.e., not used for CSA infusion) or peripheral vein. This difference, obtained when the infusion had been interrupted for 30 min, was still the case 6-12 h after discontinuation of CSA infusion. No difference was found between samples obtained from the second lumen and peripheral vein. These observations suggest that CSA might be adsorbed or bound by the silicon catheter and, thus, CSA blood level measurements may be misleading. The second lumen of the catheter or a peripheral vein should be used for accurate monitoring of CSA concentrations.
为了评估留置导管对环孢素（CSA）血药浓度测定的潜在影响，我们对8例骨髓移植受者同时从硅胶导管管腔和外周静脉采集的样本中的CSA浓度进行了评估。在静脉注射CSA停药后30分钟或6 - 12小时采集血样。通过特异性单克隆抗体测定法测定全血中的CSA浓度。用于CSA给药的管腔中的平均CSA水平显著高于从第二个管腔（即未用于CSA输注）或外周静脉采集的样本中的水平。当输注中断30分钟时出现的这种差异，在CSA输注停止6 - 12小时后仍然存在。从第二个管腔和外周静脉采集的样本之间未发现差异。这些观察结果表明，CSA可能会被硅胶导管吸附或结合，因此，CSA血药浓度测量可能会产生误导。应使用导管的第二个管腔或外周静脉来准确监测CSA浓度。
