Larsen E T, Duggan M A, Inoue M
Department of Pathology, Foothills Hospital, Calgary, Alberta, Canada.
Am J Clin Pathol. 1994 Apr;101(4):514-8. doi: 10.1093/ajcp/101.4.514.
The presence of human papilloma virus (HPV) DNA in verrucous carcinomas and some squamous-cell carcinomas (SCC) of the oropharynx suggests a role for the virus in their pathogenesis. Spindle-cell squamous carcinoma is an uncommon variant of oropharyngeal SCC. Its association with the HPV has not been reported. In situ and dot blot hybridization, using biotinylated probes to HPV 6, 11, 16, 18, 31, 33, and 35, failed to detect HPV DNA in nine studied cases of spindle-cell squamous carcinoma, as did polymerase chain reaction amplification, using a general HPV primer and type specific primers for HPV 6, 11, 16, 18, 31, and 33. Similar to previous studies, all patients were tobacco users, many were alcohol abusers, and six had a remote history of irradiation to the area. These factors, rather than the HPV, appear to have a more significant role in the development of this specific neoplasm.
口腔疣状癌和部分口咽鳞状细胞癌（SCC）中存在人乳头瘤病毒（HPV）DNA，提示该病毒在其发病机制中起一定作用。梭形细胞鳞状癌是口咽SCC的一种罕见变体。尚未见其与HPV相关性的报道。采用生物素化的HPV 6、11、16、18、31、33和35探针进行原位杂交和斑点杂交，在9例梭形细胞鳞状癌研究病例中均未检测到HPV DNA，使用通用HPV引物及HPV 6、11、16、18、31和33型特异性引物进行聚合酶链反应扩增也未检测到。与既往研究相似，所有患者均有吸烟史，许多人有酗酒史，6人有该区域既往放疗史。这些因素而非HPV，似乎在这种特定肿瘤的发生发展中起更重要作用。
