Hermann M, Kjellman H
Acta Obstet Gynecol Scand Suppl. 1976;49:1-11. doi: 10.3109/00016347609156436.
During 1968-1973 510 Rh-negative, non-Rh-immunized primi- and multigravidae giving birth to Rh-positive infants, regardless of the ABO constellation, received 250 mug immunoglobulin anti-D post partum. In serological follow-ups using the indirect Coombs' test and the papain method more than six months after childbirth, Rh-antibodies were detected in two cases with papain but not at all with the indirect Coombs' test. Seventy women gave birth to Rh-positive babies in subsequent pregnancies. Twenty-six of these non-Rh-immunized women were given an intramuscular injection of 250 mug anti-D five to ten weeks before delivery and were included in a series comprising a total of 131 Rh-negative women who had received immunoglobulin anti-D in the estimated 32nd-34th week of pregnancy. Bilirubin and haemoglobin were determined in the neonates on cord blood and serum. The infants whose mothers had received immunoglobulin anti-D during pregnancy showed no signs of haematological abnormalities related to the administration of immunoglobulin anti-D. The determination of cord blood haemoglobin or bilirubin and serum bilirubin revealed no significant difference between Rh-positive infants and Rh-negative ones. Antibodies by the papain method were detected 41 of the women at the time of delivery (22 Rh-positive babies and 19 Rh-negative ones). There was no correlation between the time at which immunoglobulin anti-D was administered and the detection of antibodies at the time of delivery, regardless of the Rh group of the infant. The indirect Coombs' test was positive only in ten of the 48 Rh-negative infants examined. 250 mug immunoglobulin anti-D provides effective post partum prophylaxis, and the same dose administered to Rh-negative pregnant women prior to delivery did not cause any detectable haemolytic damage to the fetus.
1968年至1973年期间，510名Rh阴性、未产生Rh免疫的初产妇和经产妇，无论其ABO血型组合如何，在分娩Rh阳性婴儿后均接受了250微克产后抗D免疫球蛋白注射。在产后六个多月采用间接库姆斯试验和木瓜蛋白酶法进行的血清学随访中，用木瓜蛋白酶法在两例中检测到Rh抗体，而间接库姆斯试验均未检测到。70名妇女在随后的妊娠中分娩出Rh阳性婴儿。其中26名未产生Rh免疫的妇女在分娩前5至10周接受了250微克抗D肌肉注射，并被纳入一个系列，该系列共有131名Rh阴性妇女在妊娠估计第32至34周接受了抗D免疫球蛋白注射。对新生儿的脐带血和血清进行了胆红素和血红蛋白测定。其母亲在孕期接受抗D免疫球蛋白的婴儿未表现出与抗D免疫球蛋白给药相关的血液学异常迹象。脐带血血红蛋白或胆红素以及血清胆红素的测定显示，Rh阳性婴儿和Rh阴性婴儿之间无显著差异。在分娩时，用木瓜蛋白酶法在41名妇女中检测到抗体（22名Rh阳性婴儿和19名Rh阴性婴儿）。无论婴儿的Rh血型如何，抗D免疫球蛋白的给药时间与分娩时抗体的检测之间均无相关性。在所检查的48名Rh阴性婴儿中，仅10名间接库姆斯试验呈阳性。250微克抗D免疫球蛋白提供了有效的产后预防，在分娩前给Rh阴性孕妇注射相同剂量不会对胎儿造成任何可检测到的溶血损害。
