可回收胰岛同基因移植：一种促进糖尿病大鼠手术的生物胰岛素泵

Recyclable islet isografts: a biological insulin pump to facilitate surgery in diabetic rats.

作者信息

Yang H, Yu W, Wan X, al-Abdullah I, Wright J R

机构信息

Department of Pathology, Izaak Walton Killam Children's Hospital, Dalhousie University Faculty of Medicine, Halifax, Nova Scotia, Canada.

出版信息

Cell Transplant. 1994 Jan-Feb;3(1):107-11. doi: 10.1177/096368979400300115.

DOI:10.1177/096368979400300115

PMID:8162287

Abstract

A method is described to facilitate extensive abdominal surgery in diabetic rats using temporary renal subcapsular islet isografts. The procedure is performed in three stages--each separated by 2 wk. After each rat is made diabetic (Step 1), an isogenic islet transplant is performed beneath the left renal capsule (Step 2). Step 3 is the operative procedure, followed by a left nephrectomy. Finally, it is shown how the islet isograft can be excised from the nephrectomy specimen and "recycled" into another diabetic rat (Step 2). Using this three step process, it is possible to decrease the mortality rate following orthotropic liver transplantation in diabetic rats from 100% to about 30%. This procedure can also be used to facilitate other extensive surgical procedures in diabetic rats.

摘要

描述了一种利用暂时性肾被膜下胰岛同基因移植来促进糖尿病大鼠进行广泛腹部手术的方法。该过程分三个阶段进行，每个阶段间隔2周。在每只大鼠患糖尿病后（步骤1），在左肾被膜下进行同基因胰岛移植（步骤2）。步骤3是手术操作，随后进行左肾切除术。最后，展示了如何从肾切除标本中切除胰岛移植体并“再利用”到另一只糖尿病大鼠体内（步骤2）。通过这个三步过程，可将糖尿病大鼠原位肝移植后的死亡率从100%降至约30%。该方法也可用于促进糖尿病大鼠的其他广泛外科手术。