Bulychev A G, Johansson A, Lundborg M, Camner P, Afzelius B A
Institute of Cytology of the Academy of Sciences of Russia, St. Petersburg.
Exp Mol Pathol. 1994 Feb;60(1):52-9. doi: 10.1006/exmp.1994.1005.
It is known that spermidine and other naturally occurring polyamines accumulate in rat lung slices by an energy-dependent uptake process and that alveolar macrophages (AM) have a greater rate of uptake than has the total lung cell population. In the present study rat AMs were incubated with spermidine, which resulted in a marked and significant (P < 0.002) decrease in phagocytosis of heat-killed yeast cells at concentrations 0.2 and 0.5 mM and a tendency to decrease at 0.05 mM. The number of microtubules surrounding the centrioles was measured using electron microscopy and appeared to be decreased at concentrations 0.2 and 0.5 mM. There was no affect on phagolysosomal pH. The results suggest that spermidine might affect the defense against inhaled pollutants and microbes, especially when spermidine levels are increased, as they are under conditions with high mitotic activity, e.g., in tumors.
已知亚精胺和其他天然存在的多胺通过能量依赖的摄取过程在大鼠肺切片中积累，并且肺泡巨噬细胞（AM）的摄取速率比整个肺细胞群体的摄取速率更高。在本研究中，将大鼠AMs与亚精胺一起孵育，结果发现在0.2和0.5 mM浓度下，热杀死的酵母细胞的吞噬作用显著且明显降低（P < 0.002），在0.05 mM时有一种降低的趋势。使用电子显微镜测量围绕中心粒的微管数量，发现在0.2和0.5 mM浓度下数量似乎减少。对吞噬溶酶体pH没有影响。结果表明，亚精胺可能会影响对吸入污染物和微生物的防御，特别是当亚精胺水平升高时，就像在有高有丝分裂活性的情况下，例如在肿瘤中那样。
