Didier E, Zalik S E, Didier P, Ledsham I M, Bayle D
Université de Clermont Blaise Pascal, Laboratoire d'Immunologie G, Aubière, France.
Histochemistry. 1993 Dec;100(6):485-93. doi: 10.1007/BF00267830.
The location of soluble lactose-binding proteins (S-lac lectins) has been studied by immunohistochemical methods during morphogenesis of the chick embryo, when segregation and early differentiation of organ primordia was occurring. Using a panel of polyclonal antisera raised to various purified lectin preparations, we observed striking differences in the antigenic properties of these antisera, indicating that diverse versions of the lectins may be expressed during development. The antisera referred to as anti-L-16, anti-M-16, anti-S-14 and anti-I-14 were respectively raised to native or denatured 16 kDa lectins from adult liver and embryonic muscle and to 14 kDa lectins from embryonic skin and adult intestine. Having determined the optimal immunohistochemical conditions in the preparation of embryo sections (fixation, embedding, sectioning) we show that anti-L-16, anti-S-14 and anti-I-14 mostly bind the lectins expressed at the cell surface, in the extracellular matrix and in some released secretion. As previously shown, anti-L-16 and anti-S-14 are also able to recognize the cytoplasmic form of some migrative lectin-rich cells (primitive streak, neural crest cells, germ cells). Anti-M-16 was bound exclusively to the cytoplasmic form of the 16 kDa lectin in the same cell lines as above and also in some others, such as in the notochord, the myotomal part of the somites, the pharyngeal endoderm and the cardiac muscle. These different antigenic properties may be applied to the accurate mapping of various lectin isoforms and evaluation of the respective contribution of their intra- and extracellular variants during development and differentiation.
在鸡胚形态发生过程中，当器官原基正在分离和早期分化时，我们采用免疫组织化学方法研究了可溶性乳糖结合蛋白（S-乳糖凝集素）的定位。使用针对各种纯化凝集素制剂制备的一组多克隆抗血清，我们观察到这些抗血清在抗原特性上存在显著差异，这表明在发育过程中可能表达了凝集素的不同变体。分别称为抗L-16、抗M-16、抗S-14和抗I-14的抗血清，是针对来自成年肝脏和胚胎肌肉的天然或变性16 kDa凝集素以及来自胚胎皮肤和成年肠道的14 kDa凝集素制备的。在确定胚胎切片制备中的最佳免疫组织化学条件（固定、包埋、切片）后，我们发现抗L-16、抗S-14和抗I-14大多结合在细胞表面、细胞外基质和一些分泌的分泌物中表达的凝集素。如先前所示，抗L-16和抗S-14也能够识别一些富含凝集素的迁移细胞（原条、神经嵴细胞、生殖细胞）的细胞质形式。抗M-16仅与上述相同细胞系以及其他一些细胞系（如脊索、体节的肌节部分、咽内胚层和心肌）中16 kDa凝集素的细胞质形式结合。这些不同的抗原特性可用于准确绘制各种凝集素同工型图谱，并评估其细胞内和细胞外变体在发育和分化过程中的各自贡献。
