O'Connell A C, Pearson S K, Bowen W H
Department of Dental Research, Rochester Caries Research Center, University of Rochester, New York 14642.
J Dent Res. 1994 Mar;73(3):637-43. doi: 10.1177/00220345940730030901.
This study examined the effect of pilocarpine on caries and saliva composition in rats with compromised salivary gland function. Eight litters of specific-pathogen-free female Sprague-Dawley rats were divided into five groups for surgery. Partial desalivation was performed in either of three ways: (1) Both parotid ducts were ligated; (2) both submandibular/sublingual (SM/SL) glands were excised, or (3) one parotid duct was ligated, and submandibular/sublingual glands were removed unilaterally. Completely desalivated animals and unoperated animals served as positive and negative controls, respectively. One week following surgery, groups were subdivided so that half of each group had osmotic pumps implanted which delivered pilocarpine HCl (24 mg/kg/day). All animals were infected with Streptococcus sobrinus 6715 and fed cariogenic diet ad libitum for 28 days. Caries scores, microbiological data, and saliva flow rate and composition were determined for all animals. Animals which had both SM/SL glands removed and received pilocarpine developed significantly lower sulcal caries (p < or = 0.05) compared with the animals that had both SM/SL removed but did not receive pilocarpine. The concentration of protein in parotid saliva in these pilocarpine-treated animals was unaffected, and no differences were observed in the electrophoretic profiles on SDS-PAGE. Pilocarpine appears to exert its greatest caries-protective effect when the parotid glands remain intact.
本研究考察了毛果芸香碱对唾液腺功能受损大鼠龋齿及唾液成分的影响。将8窝无特定病原体的雌性斯普拉格-道利大鼠分为5组进行手术。采用三种方法之一进行部分去唾液腺处理：（1）结扎双侧腮腺导管；（2）切除双侧下颌下/舌下（SM/SL）腺，或（3）结扎一侧腮腺导管，并单侧切除下颌下/舌下腺。完全去唾液腺的动物和未手术的动物分别作为阳性和阴性对照。手术后1周，将每组再分为两半，使每组的一半植入渗透泵，输送盐酸毛果芸香碱（24 mg/kg/天）。所有动物均感染远缘链球菌6715，并随意喂食致龋饮食28天。测定所有动物的龋齿评分、微生物学数据以及唾液流速和成分。与切除双侧SM/SL腺但未接受毛果芸香碱治疗的动物相比，切除双侧SM/SL腺并接受毛果芸香碱治疗的动物沟裂龋显著降低（p≤0.05）。这些接受毛果芸香碱治疗的动物腮腺唾液中的蛋白质浓度未受影响，SDS-PAGE电泳图谱也未观察到差异。当腮腺保持完整时，毛果芸香碱似乎发挥其最大的防龋作用。
